Demonstrators on Wednesday promised human shields to protect the animals.
Beatle Paul McCartney stars in an international campaign against a planned cull of hundreds of kangaroos on an Australian military base.
McCartney appeared on a website set up by the British animal welfare group Viva! to condemn the cull of up to 500 animals using tranquilizer darts and lethal injection.
"There is an urgent need for action to protect kangaroos from a barbaric industry which slaughters them for meat and leather," McCartney said in an undated message.
"Please do all you can to help Viva! end this shameful massacre."
The eastern grey kangaroos, which feature on Australia's coat of arms, are living on a military communications base in the nation's capital Canberra.
Authorities say the animals, on death row since May last year, threaten other local species through overgrazing.
Viva!, or Vegetarians International Voice for Animals, said it had launched a Europe-wide campaign against the cull and by Wednesday had gathered more than 1,300 protest signatures from 36 countries on an Australian-based web page.